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1. (WO2018205037) TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND OTHER INDUSTRIALLY USEFUL YEAST

Pub. No.:    WO/2018/205037    International Application No.:    PCT/CA2018/050569
Publication Date: Fri Nov 16 00:59:59 CET 2018 International Filing Date: Tue May 15 01:59:59 CEST 2018
IPC: C12N 15/81
C07C 59/08
C07C 59/235
C07C 59/245
C07C 15/113
C12P 7/46
C12P 7/56
Applicants: BIOAMBER INC.
Inventors: RYAN, Owen
Title: TOOLS AND METHODS FOR GENOME EDITING ISSATCHENKIA ORIENTALIS AND OTHER INDUSTRIALLY USEFUL YEAST
Abstract:
The present description relates to genetic tools and methods to facilitate transformation and genetic engineering of industrially-useful yeast/fungal species, such as Issatchenkia orientalis, for which a robust set of genetic tools such as stably inherited and maintained plasmids and functional control sequences is presently lacking. Thus, the present description relates to autonomously replicating sequences (ARSs), RNA polymerase II/III promoters, RNA polymerase II/III terminators, expression cassettes, and vectors comprising same are described herein, as well as uses and methods relating to same, which are functional in I. orientalis.