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1. (WO2018204986) ENCAPSULATION OF EUKARYOTIC CELLS FOR CELLULAR SCREENING OF EXPRESSED SEQUENCES

Pub. No.:    WO/2018/204986    International Application No.:    PCT/AU2018/050442
Publication Date: Fri Nov 16 00:59:59 CET 2018 International Filing Date: Sat May 12 01:59:59 CEST 2018
IPC: C40B 20/00
C40B 30/00
C08F 2/48
Applicants: THE FLOREY INSTITUTE OF NEUROSCIENCE AND MENTAL HEALTH
Inventors: SCOTT, Daniel James
BATHGATE, Ross
Title: ENCAPSULATION OF EUKARYOTIC CELLS FOR CELLULAR SCREENING OF EXPRESSED SEQUENCES
Abstract:
Provided herein are methods for selecting polypeptides or proteins having one or more desired properties from a library of sequences expressed in eukaryotic cells, comprising: encapsulating said cells by photopolymerization; solubilizing said encapsulated cells to produce semipermeable microcapsules; optionally contacting said cells and/or said microcapsules with one or more agents to facilitate detection of activity or function of polypeptides or proteins of interest; and selecting polypeptides or proteins of interest having one or more desired properties. Also provided are methods for encapsulating eukaryotic cells for use in the selection of polypeptides and proteins as described above.