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1. (WO2018204854) METHODS OF PREPARING A RE-USABLE SINGLE CELL AND METHODS FOR ANALYZING THE EPIGENOME, TRANSCRIPTOME, AND GENOME OF A SINGLE CELL
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Pub. No.: WO/2018/204854 International Application No.: PCT/US2018/031201
Publication Date: 08.11.2018 International Filing Date: 04.05.2018
IPC:
C12N 11/04 (2006.01) ,G01N 1/28 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11
Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02
Enzymes or microbial cells being immobilised on or in an organic carrier
04
entrapped within the carrier, e.g. gel, hollow fibre
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
Applicants:
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES [US/US]; Office of Technology Transfer National Institutes of Health 6011 Executive Boulevard, Suite 325 MSC 7660, Bethesda, Maryland 20892-7660, US
Inventors:
TOSATO, Giovanna; US
OHNUKI, Hidetaka; US
Agent:
LAWLEY, Stephanie M.; US
LEYDIG, VOIT & MAYER, LTD.; Leydig, Voit & Mayer, Ltd. Two Prudential Plaza, Suite 4900 180 North Stetson Avenue Chicago, Illinois 60601, US
Priority Data:
62/502,24705.05.2017US
Title (EN) METHODS OF PREPARING A RE-USABLE SINGLE CELL AND METHODS FOR ANALYZING THE EPIGENOME, TRANSCRIPTOME, AND GENOME OF A SINGLE CELL
(FR) PROCÉDÉS DE PRÉPARATION D'UNE CELLULE ISOLÉE RÉUTILISABLE ET PROCÉDÉS D'ANALYSE DE L'ÉPIGÉNOME, DU TRANSCRIPTOME ET DU GÉNOME D'UNE CELLULE ISOLÉE
Abstract:
(EN) Methods and kits for preparing re-usable single cells are described. Cell components are anchored using a nano-scale scaffold to create a re-usable single cell. The nano-scale scaffold may be a polyacrylamide nano-scale scaffold. Methods to determine modifications of the genome, transcriptome, or epigenome are described.
(FR) L'invention concerne des procédés et des kits de préparation de cellules isolées réutilisables. Des composants cellulaires sont ancrés à l'aide d'un échafaudage nanométrique pour créer une cellule isolée réutilisable. L'échafaudage nanométrique peut être un échafaudage nanométrique de polyacrylamide. L'invention concerne des procédés pour déterminer des modifications du génome, du transcriptome ou de l'épigénome.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)