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1. (WO2018204814) A METHOD TO KEEP THE EXCITATION LIGHT SHEET IN FOCUS IN SELECTIVE PLANE ILLUMINATION MICROSCOPY

Pub. No.:    WO/2018/204814    International Application No.:    PCT/US2018/031132
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Sat May 05 01:59:59 CEST 2018
IPC: G02B 21/24
G02B 21/16
G02B 21/36
Applicants: INTELLIGENT IMAGING INNOVATIONS, INC.
Inventors: GAO, Liang
Title: A METHOD TO KEEP THE EXCITATION LIGHT SHEET IN FOCUS IN SELECTIVE PLANE ILLUMINATION MICROSCOPY
Abstract:
Keeping the excitation light sheet in focus is critical in selective plane illumination microscopy (SPIM) to ensure its 3D imaging ability. Unfortunately, an effective method that can be used in SPIM on general biological specimens to find the axial position of the excitation light sheet and keep it in focus is barely available. Here, we present a method to solve the problem. We investigate its mechanism and demonstrate its performance on a lattice light sheet microscope.