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1. (WO2018204367) PROFILING OF DNA METHYLATION USING MAGNETORESISTIVE BIOSENSOR ARRAY

Pub. No.:    WO/2018/204367    International Application No.:    PCT/US2018/030457
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Wed May 02 01:59:59 CEST 2018
IPC: C12Q 1/68
G01N 33/53
G01N 33/543
Applicants: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
DANMARKS TEKNISKE UNIVERSITET
Inventors: LEE, Jung-Rok
WANG, Shan, X.
HANSEN, Mikkel, F.
DUFVA, Martin
RIZZI, Giovanni
Title: PROFILING OF DNA METHYLATION USING MAGNETORESISTIVE BIOSENSOR ARRAY
Abstract:
A method of methylation detection provides a quantitative description of the methylation density in DNA strands. Bisulphite conversion [100] of the DNA strands containing methylated and unmethylated sites creates converted DNA strands with mismatch base pairs. The converted DNA strands are PCR amplified [102], and single strand target DNA strands are magnetically labeled [104] and hybridized [106] with complementary DNA strands immobilized onto a magnetoresistive (MR) sensor array. During hybridization, a binding signal may be recorded. A stringency condition such as temperature or salt concentration is increased [108] to cause the magnetically labeled single strand target DNA strands to be denatured from the complementary DNA strands immobilized onto a magnetoresistive (MR) sensor array. During the increasing of the stringency condition a denaturation signal resulting from the denatured magnetically labeled single strand target DNA strands is recorded [110] in real time and used to determine [112] stringency conditions of methylated and unmethylated DNA strands. The DNA strands may also contain wild type genes and mutated genes, so that mutation sites may be determined simultaneously with methylation sites.