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1. (WO2018204314) HIGH YIELDS OF ISOMELEZITOSE FROM SUCROSE BY ENGINEERED GLUCANSUCRASES

Pub. No.:    WO/2018/204314    International Application No.:    PCT/US2018/030373
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Wed May 02 01:59:59 CEST 2018
IPC: C12N 9/10
C12N 15/52
C12P 19/02
Applicants: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE
Inventors: COTE, Gregory, L.
SKORY, Christopher, D.
Title: HIGH YIELDS OF ISOMELEZITOSE FROM SUCROSE BY ENGINEERED GLUCANSUCRASES
Abstract:
Provided herein are compositions and methods for the synthesis of the trisaccharide, isomelezitose, using genetically modified glucansucrase enzymes from representative microorganisms, including lactic acid bacteria such as Leuconostoc mesenteroides. Various modified enzymes are detailed, increasing isomelezitose yields and provide the foundation for large-scale production of isomelezitose for food, industrial and biomedical applications.