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1. (WO2018204135) METHODS OF DETERMINING PROTEIN STRUCTURE USING TWO-PHOTON FLUORESCENCE MEASUREMENTS

Pub. No.:    WO/2018/204135    International Application No.:    PCT/US2018/029234
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Wed Apr 25 01:59:59 CEST 2018
IPC: G01N 33/53
Applicants: BIODESY, INC.
Inventors: CLANCY, Bason
SALAFSKY, Joshua
Title: METHODS OF DETERMINING PROTEIN STRUCTURE USING TWO-PHOTON FLUORESCENCE MEASUREMENTS
Abstract:
Methods, devices, and systems for using two-photon fluorescence measurements, either alone or in combination with other nonlinear optical measurements such as second harmonic generation, sum frequency generation, or difference frequency generation, to determine structural parameters such as mean tilt angle and distribution width for tethered nonlinear-active biomolecules are described. The disclosed methods, devices, and systems may also be used to perform structural comparisons of two or more biomolecular samples; to detect changes in biomolecule conformation upon binding of a ligand; and to screen candidate binding partners to identify compounds that modulate the conformation of the biomolecule.