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1. (WO2018203541) METHOD OF RECOVERING AND PURIFYING POLYPEPTIDES HAVING IMMUNOGLOBULIN BINDING ACTIVITY

Pub. No.:    WO/2018/203541    International Application No.:    PCT/JP2018/017326
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Sat Apr 28 01:59:59 CEST 2018
IPC: C07K 1/14
B01D 15/00
B01D 15/12
B01D 15/38
B01J 20/281
C12P 21/02
G01N 30/88
B01J 20/24
C07K 14/00
C07K 16/00
C12N 15/09
Applicants: KANEKA CORPORATION
株式会社カネカ
Inventors: KARASUGI, Keiichi
唐杉 慶一
FUNAKI, Masahiro
船木 正大
NISHIHACHIJO, Masakatsu
西八條 正克
NAKANO, Yoshiyuki
中野 喜之
MINAKUCHI, Kazunobu
水口 和信
Title: METHOD OF RECOVERING AND PURIFYING POLYPEPTIDES HAVING IMMUNOGLOBULIN BINDING ACTIVITY
Abstract:
The present invention is characterized by providing a treatment method for recovering and purifying polypeptides having immunoglobulin binding activity using a genetically modified organism, wherein, rather than relying on differences in polypeptide host or amino acid sequence, impurities comprising host-originating cell components, contaminant proteins, and nucleic acids are precipitated at high efficiency to a larger particle size, thereby allowing for improved filterability and improved productivity in chromatography processes. This purifying method for polypeptides having immunoglobulin binding activity is characterized by comprising: (1) a step for culturing a genetically modified organism that produces polypeptides having immunoglobulin binding activity; (2) a step for performing an acidic heat treatment upon one or more of culture liquid obtained in step (1), supernatant therefrom, a liquid suspension of microbes in the culture liquid, crushed microbe liquid obtained by crushing the microbes in the liquid microbe suspension, and crushed microbe supernatant obtained by removing the microbes from the crushed microbe liquid; and (3) a step for separating the precipitate.