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1. (WO2018202911) RAPID PURIFICATION OF HIGH QUALITY NUCLEIC ACIDS FROM BIOLOGICAL SAMPLES

Pub. No.:    WO/2018/202911    International Application No.:    PCT/EP2018/061695
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Tue May 08 01:59:59 CEST 2018
IPC: C12N 15/10
Applicants: BIOECHO LIFE SCIENCES GMBH
Inventors: MÜLLER, Markus
HUCKLENBROICH, Jörg
Title: RAPID PURIFICATION OF HIGH QUALITY NUCLEIC ACIDS FROM BIOLOGICAL SAMPLES
Abstract:
This invention relates to a method for the purification of nucleic acids, preferably DNA, from biological samples, comprising the steps (a) optional lysis of said sample, (b) optional heat incubation of said sample, (c) enzymatic digestion of non-nucleic acid components in the product of step (a) or (b), (d) heat inactivation of one or more enzyme(s) used in step (c), (e) transfer of the product of step (d) onto a resin capable of retaining non-nucleic acid components, while the nucleic acids pass through the resin, thereby purifying the nucleic acids.