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1. (WO2018202894) CELL CULTURE DEVICE

Pub. No.:    WO/2018/202894    International Application No.:    PCT/EP2018/061604
Publication Date: Fri Nov 09 00:59:59 CET 2018 International Filing Date: Sat May 05 01:59:59 CEST 2018
IPC: C12M 1/34
C12M 1/00
C12M 3/00
Applicants: UNIVERSITÄT ZÜRICH
NTNU - NORGES TEKNISK-NATURVITENSKAPELIGE UNIVERSITET
Inventors: KRIESI, Carlo
KURTCUOGLU, Vartan
MARMARAS, Anastasios
STEINERT, Martin
Title: CELL CULTURE DEVICE
Abstract:
The present invention relates to a cell culture device (1) for use with an optical microscope (40), comprising a housing (10) that is configured to be placed onto a stage of an optical microscope (40) in front of an objective (41) of the microscope (40), the housing (10) enclosing an internal space (11) of the housing (10), a removable flow chamber (2) enclosing an internal space (20) for accommodating a cell culture (CC) comprising living biological cells, a heater (3) arranged in the internal space (11) of the housing (10) for heating said fluid medium (M) to be guided through the flow chamber (2), a first flow path (P1) arranged in the internal space (11) of the housing (10) for guiding said fluid medium (M) towards the flow chamber (2) via said heater (3), and a second flow path (P2) arranged in the internal space (11) of the housing (2) for guiding said fluid medium (M) away from the flow chamber (2), and a pump (4) for pumping said fluid medium (M) through the first flow path (P1) into the internal space (20) of the flow chamber (2) and through the second flow path (P2) out of the internal space (20) of the flow chamber (2) when the flow chamber (2) is inserted into the internal space (11) of the housing (10).