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1. (WO2018201153) TARGETED GENE DISRUPTION METHODS AND IMMUNOGENIC COMPOSITIONS

Pub. No.:    WO/2018/201153    International Application No.:    PCT/US2018/030302
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Tue May 01 01:59:59 CEST 2018
IPC: A61K 35/74
A61K 39/00
A61K 39/118
C12N 1/20
C12N 1/21
C12N 15/09
Applicants: KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Inventors: GANTA, Roman R.
WANG, Ying
Title: TARGETED GENE DISRUPTION METHODS AND IMMUNOGENIC COMPOSITIONS
Abstract:
Targeted disruption of a specific gene and its subsequent restoration in obligate intracellular bacteria remains extremely challenging due to their absolute requirement for residence inside a host cell to replicate. Here, targeted allelic exchange mutations were created to inactivate two genes and then to restore one of the two genes of a rickettsial pathogen. Ehrlichia chaffeensis. These methods were then also successfully utilized in Ehrlichia canis and Anaplasma phagocyophilum. The resultant mutated pathogens are useful in immunogenic compositions for reducing the incidence of or severity of infection with ricksettsial pathogens.