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1. (WO2018200872) BIOLOGICAL PROCESS SYSTEMS AND METHODS USING MICROFLUIDIC APPARATUS HAVING AN OPTIMIZED ELECTROWETTING SURFACE

Pub. No.:    WO/2018/200872    International Application No.:    PCT/US2018/029648
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Fri Apr 27 01:59:59 CEST 2018
IPC: B01L 3/00
Applicants: BERKELEY LIGHTS, INC.
Inventors: MCEWEN, Jason M.
SOUMILLON, Magali
PEI, Shao Ning
LOWE, JR., Randall D.
NEDUNGADI, Samira A.
KURZ, Volker L.S.
GONG, Jian
MEJIA GONZALEZ, Yara X.
TOH, Mckenzi S.
RABKIN, Brian A.
BRIGGS, Jason C.
KELLY-GREENE, Darcy K.
PORTER, JR., James M.
Title: BIOLOGICAL PROCESS SYSTEMS AND METHODS USING MICROFLUIDIC APPARATUS HAVING AN OPTIMIZED ELECTROWETTING SURFACE
Abstract:
Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided for processing biological cells, e.g., for use in nucleic acid library preparation and/or synthesis (including amplification). The devices include a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode. Methods of nucleic acid library preparation and/or synthesis can involve providing reagents to cells or nucleic acids by merging appropriate droplets on a droplet actuation surface within a water-immiscible organic liquid and can be performed in the presence of appropriate surfactants. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are systems for temperature control of the microfluidic device during nucleic acid library preparation and/or synthesis which can reduce temperature overshooting during heating and cooling steps.