Search International and National Patent Collections
|1. (WO2018200669) INDUCING MICROGLIA FROM HEMATOPOIETIC AND PLURIPOTENT STEM CELLS|
|Applicants:||CHILDREN'S MEDICAL CENTER CORPORATION
|Title:||INDUCING MICROGLIA FROM HEMATOPOIETIC AND PLURIPOTENT STEM CELLS|
Provided herein are robust and scalable methods of generating microglia-like cells (e.g., in vitro or ex vivo). The methods involve differentiating a cell (e.g., an iPS, a HSC, or a CD34+ cell) into a monocyte, and differentiating the monocyte into a microglia-like cell. In some embodiments, the methods further comprises expanding the cell prior to differentiation, thus enabling the generation of a large number of microglia-like cells from a smaller number of cells. The microglia-like cells generated using the methods described herein are homogenous and maintain the biological activities and functionalities of natural microglia cells.