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1. (WO2018200669) INDUCING MICROGLIA FROM HEMATOPOIETIC AND PLURIPOTENT STEM CELLS

Pub. No.:    WO/2018/200669    International Application No.:    PCT/US2018/029354
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Thu Apr 26 01:59:59 CEST 2018
IPC: C12N 5/071
C12N 5/0789
C12N 5/0786
C12N 5/00
C12N 5/079
C12N 5/0735
C12N 5/0797
G01N 33/53
Applicants: CHILDREN'S MEDICAL CENTER CORPORATION
Inventors: ROSSI, Derrick
PAULSEN, Bruna
NAGAI, Manavi
Title: INDUCING MICROGLIA FROM HEMATOPOIETIC AND PLURIPOTENT STEM CELLS
Abstract:
Provided herein are robust and scalable methods of generating microglia-like cells (e.g., in vitro or ex vivo). The methods involve differentiating a cell (e.g., an iPS, a HSC, or a CD34+ cell) into a monocyte, and differentiating the monocyte into a microglia-like cell. In some embodiments, the methods further comprises expanding the cell prior to differentiation, thus enabling the generation of a large number of microglia-like cells from a smaller number of cells. The microglia-like cells generated using the methods described herein are homogenous and maintain the biological activities and functionalities of natural microglia cells.