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1. (WO2018200386) COMPOSITIONS AND METHODS FOR IMPROVING SAMPLE IDENTIFICATION IN INDEXED NUCLEIC ACID LIBRARIES

Pub. No.:    WO/2018/200386    International Application No.:    PCT/US2018/028881
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Tue Apr 24 01:59:59 CEST 2018
IPC: C12Q 1/6806
C12Q 1/6855
Applicants: ILLUMINA, INC.
ILLUMINA CAMBRIDGE LIMITED
Inventors: VERMAAS, Eric, Hans
KHOSROHEIDARI, Mahdieh
BEVIS-MOTT, Calire
Title: COMPOSITIONS AND METHODS FOR IMPROVING SAMPLE IDENTIFICATION IN INDEXED NUCLEIC ACID LIBRARIES
Abstract:
The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5' and 3' ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.