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1. (WO2018200290) PRODUCTION OF HIGH PURITY CHONDROITINASE ABC
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Pub. No.: WO/2018/200290 International Application No.: PCT/US2018/028214
Publication Date: 01.11.2018 International Filing Date: 19.04.2018
IPC:
C12N 9/88 (2006.01) ,B01D 15/38 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08
Selective adsorption, e.g. chromatography
26
characterised by the separation mechanism
38
involving specific interaction not covered by one or more of groups B01D15/30-B01D15/36120
Applicants:
ADVANTEK SERUM LABORATORIES LTD.; Flat L, 8/F, On Wah Industrial Building, 41-43 Au Pui Wan Street, Fotan, Shatin Hong Kong, CN
Inventors:
WONG, Bing Lou; US
CHUNG, Wai Hong; CN
Agent:
BURKE, Margaret; CN
YIP, Sam; HK
Priority Data:
15/494,58924.04.2017US
15/788,82420.10.2017US
201710271643.324.04.2017CN
Title (EN) PRODUCTION OF HIGH PURITY CHONDROITINASE ABC
(FR) PRODUCTION DE CHONDROÏTINASE ABC DE HAUTE PURETÉ
Abstract:
(EN) The present invention provides a method for purifying Chondroitinase ABC (ChABC). The present method includes using a heparin-immobilized affinity chromatography column, and through chromatography method obtaining a purified ChABC from a matrix containing said ChABC. The present method is capable of obtaining ChABC in high purity with the advantages of simplicity in preparation and high yield.
(FR) La présente invention concerne un procédé de purification de chondroïtinase ABC (ChABC). Le présent procédé consiste à utiliser une colonne de chromatographie d'affinité immobilisée sur de l'héparine, et à obtenir, au moyen du procédé de chromatographie, une ChABC purifiée à partir d'une matrice contenant ladite ChABC. Le présent procédé permet d'obtenir une ChABC de haute pureté avec comme avantages une simplicité de préparation et un rendement élevé.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)