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1. (WO2018199840) MICROFLUIDIC METHOD AND SYSTEM FOR DETECTING BASOPHIL ALLER-GEN THRESHOLD SENSITIVITY (CD-SENS) BY USING A GRADIENT OF AT LEAST ONE ALLERGEN
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Pub. No.: WO/2018/199840 International Application No.: PCT/SE2018/050419
Publication Date: 01.11.2018 International Filing Date: 24.04.2018
IPC:
C07K 16/18 (2006.01) ,C12M 1/34 (2006.01) ,C12Q 1/02 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/567 (2006.01) ,G01N 33/569 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
566
using specific carrier or receptor proteins as ligand binding reagent
567
utilising isolate of tissue or organ as binding agent
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
Applicants:
RUSSOM, Amam [SE/SE]; SE
NOPP SCHERMAN, Anna [SE/SE]; SE
LUNDAHL, Joachim [SE/SE]; SE
JOHANSSON, Gunnar [SE/SE]; SE
Inventors:
RUSSOM, Amam; SE
NOPP SCHERMAN, Anna; SE
LUNDAHL, Joachim; SE
JOHANSSON, Gunnar; SE
Agent:
BERGENSTRÅHLE & PARTNERS STOCKHOLM AB; Box 17704 118 93 Stockholm, SE
Priority Data:
1750500-925.04.2017SE
Title (EN) MICROFLUIDIC METHOD AND SYSTEM FOR DETECTING BASOPHIL ALLER-GEN THRESHOLD SENSITIVITY (CD-SENS) BY USING A GRADIENT OF AT LEAST ONE ALLERGEN
(FR) PROCÉDÉ ET SYSTÈME MICROFLUIDIQUES POUR LA DÉTECTION DE LA SENSIBILITÉ SEUIL D'ALLERGÈNES BASOPHILES (CD-SENS) À L'AIDE D'UN GRADIENT D'AU MOINS UN ALLERGÈNE
Abstract:
(EN) The present invention relates to a microfluidic based method and system for detec- tion of basophil allergen threshold sensitivity (CD-sens). The method and system in- volves taking whole blood directly from the allergic patient and isolate the basophils for activation followed by image based detection of the degranulation markers. The method and system involves the isolation of basophils which express CD203c sur- face markers direct from whole blood, activating the isolated basophils with at least one activation composition comprising a gradient of anti-Fcɛ RI or a gradient of at least one allergen, capturing the activated basophils and detection of expression of CD63 degranulation markers expressed on the basophils by using microscopy.
(FR) La présente invention concerne un procédé et un système à base de microfluides pour la détection de la sensibilité seuil d'allergènes basophiles (CD-sens). Le procédé et le système de l'invention consistent à prélever du sang total directement à partir d'un patient allergique et à isoler des basophiles pour une activation suivie d'une détection à base d'image de marqueurs de dégranulation. Le procédé et le système de l'invention consistent également à isoler des basophiles exprimant des marqueurs de surface CD203c directement à partir du sang total, activer des basophiles isolés avec au moins une composition d'activation comprenant un gradient d'anti-Fcɛ RI ou un gradient d'au moins un allergène, capturer des basophiles activés et détecter l'expression de marqueurs de dégranulation CD63 exprimés sur des basophiles en utilisant la microscopie.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)