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1. (WO2018199279) METHOD FOR DETERMINING LIFE OR DEATH OF MICROORGANISM USING RIBOSOMAL RNA PRECURSOR

Pub. No.:    WO/2018/199279    International Application No.:    PCT/JP2018/017145
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Sat Apr 28 01:59:59 CEST 2018
IPC: C12N 15/09
C12M 1/34
C12Q 1/686
C12Q 1/6876
G01N 33/02
Applicants: NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
国立大学法人東京医科歯科大学
Inventors: SHIMIZU Norio
清水 則夫
WATANABE Ken
渡邊 健
MORIO Tomohiro
森尾 友宏
Title: METHOD FOR DETERMINING LIFE OR DEATH OF MICROORGANISM USING RIBOSOMAL RNA PRECURSOR
Abstract:
The present invention addresses the problem of providing a means whereby life or death of microbial species over a broad range can be quickly and exactly determined. Provided are: an oligonucleotide set for determining life or death of a microorganism, said set comprising oligonucleotides designed in two ribosomal RNA gene regions between which the spacer region of a ribosomal RNA precursor (pre-rRNA) is sandwiched; a kit for determining life or death of a microorganism, said kit comprising the oligonucleotide set; and a method for determining life or death of a microorganism using the oligonucleotide set.