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1. (WO2018199142) METHOD FOR PRODUCING NEURAL CREST CELLS AND SYMPATHETIC NEURONS

Pub. No.:    WO/2018/199142    International Application No.:    PCT/JP2018/016758
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Thu Apr 26 01:59:59 CEST 2018
IPC: C12N 5/079
A61K 35/30
A61K 35/545
A61L 27/38
A61P 25/02
C12N 5/0775
C12N 5/0793
Applicants: KYOTO UNIVERSITY
国立大学法人京都大学
Inventors: NAKAHATA, Tatsutoshi
中畑 龍俊
SAITO, Megumu
齋藤 潤
KIRINO, Kosuke
桐野 浩輔
Title: METHOD FOR PRODUCING NEURAL CREST CELLS AND SYMPATHETIC NEURONS
Abstract:
Pluripotent stem cells are cultured in a culture solution containing FGF2, retinoic acid and BMP4 to thereby produce neural crest cells. The neural crest cells are cultured in a culture solution containing a cytokine and BMP4 to thereby produce sympathetic neuron precursors. Further, the sympathetic neuron precursors are cultured in a culture solution containing a neurotrophic factor to thereby produce sympathetic neurons.