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1. (WO2018199137) METHOD FOR DETECTING MINOR BCR-ABL1 GENE

Pub. No.:    WO/2018/199137    International Application No.:    PCT/JP2018/016749
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Thu Apr 26 01:59:59 CEST 2018
IPC: C12Q 1/686
C12N 15/09
C12Q 1/6886
Applicants: OTSUKA PHARMACEUTICAL CO., LTD.
大塚製薬株式会社
Inventors: KATSURAGI, Kiyonori
葛城 粛典
TANAKA, Hideaki
田中 秀明
ITO, Ryuta
伊藤 隆太
KOGA, Daisuke
古賀 大輔
Title: METHOD FOR DETECTING MINOR BCR-ABL1 GENE
Abstract:
The present invention provides a method including: (1) a step for performing PCR using a forward primer having a portion of the base sequence of exon 1 of the BCR gene and a reverse primer having a base sequence complementary to a portion of exons 2 through 11 of the ABL1 gene, with a nucleic acid sample obtained from a subject as a template, in the presence of a modified nucleic acid having the base sequence of a portion of exons 2 through 14 of the BCR gene or a base sequence complementary thereto; and (2) a step for determining that the subject has the minor BCR-ABL1 gene in the case that a nucleic acid is amplified by PCR.