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1. (WO2018199136) METHOD FOR MEASURING EXPRESSION LEVEL OF ABL1 T315I MUTATION

Pub. No.:    WO/2018/199136    International Application No.:    PCT/JP2018/016748
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Thu Apr 26 01:59:59 CEST 2018
IPC: C12Q 1/6883
C12N 15/62
Applicants: OTSUKA PHARMACEUTICAL CO., LTD.
大塚製薬株式会社
Inventors: KATSURAGI, Kiyonori
葛城 粛典
TANAKA, Hideaki
田中 秀明
ITO, Ryuta
伊藤 隆太
KOGA, Daisuke
古賀 大輔
Title: METHOD FOR MEASURING EXPRESSION LEVEL OF ABL1 T315I MUTATION
Abstract:
This disclosure provides a method that contains (1) a step for reverse transcribing a target RNA specimen in the presence of a modified nucleic acid having a base sequence complementary to a region that contains the T315I mutation position of wild-type ABL1 mRNA, using, in the same container, (a) a reverse primer that binds to a region downstream from the T315I mutation position of ABL1 mRNA and (b) a reverse primer that binds to a region upstream from the T315I mutation position of ABL1 mRNA, and (2) a step for determining the expression level of the ABL1 T315I mutation on the basis of the ratio of the reverse transcription product due to the primer (a) with respect to the reverse transcription product due to the primer (b). This disclosure also provides a kit for this method.