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1. (WO2018199106) FROZEN BIOLOGICAL CELL PELLET PREPARATION DEVICE
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Pub. No.: WO/2018/199106 International Application No.: PCT/JP2018/016651
Publication Date: 01.11.2018 International Filing Date: 24.04.2018
IPC:
C12M 1/00 (2006.01) ,A01N 1/02 (2006.01) ,C12N 5/076 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1
Preservation of bodies of humans or animals, or parts thereof
02
Preservation of living parts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
076
Sperm cells; Spermatogonia
Applicants:
株式会社北里コーポレーション KITAZATO CORPORATION [JP/JP]; 静岡県富士市中島81番地 81, Nakajima, Fuji-shi, Shizuoka 4160907, JP
フォンダシオン イヴィ FUNDACION IVI [ES/ES]; バレンシア アベニーダ フェルナンド アブリル マルトレル 106 トッレ ア ビオポロ ラフェ ヘルス リセルチ インスティテュート オスピタル ラフェ Biopolo La Fe Health Research Institute Hospital La Fe Avenida Fernando Abril Martorell 106 Torre A Valencia 46026, ES
Inventors:
井上 太 INOUE Futoshi; JP
パボン レアルプ ディーナ  PABON REALPE Dina; ES
デ ロ サントス モーリーナ マリア ホセ  DE LOS SANTOS MOLINA Maria Jose; ES
コボ カバール アナ  COBO CABAL Ana; ES
メセゲー エスクリーバ マルコス  MESEGUER ESCRIVA Marcos; ES
ペライサー マーティネ アントーニオ  PELLICER MARTINEZ Antonio; ES
レモイ ヒメネ ホセ  REMOHI GIMENEZ Jose; ES
Agent:
向山 正一 MUKAIYAMA Shoichi; JP
Priority Data:
2017-08806827.04.2017JP
Title (EN) FROZEN BIOLOGICAL CELL PELLET PREPARATION DEVICE
(FR) DISPOSITIF DE PRÉPARATION DE CULOT DE CELLULES BIOLOGIQUES CONGELÉES
(JA) 生体細胞粒状凍結体作成具
Abstract:
(EN) A frozen biological cell pellet preparation device 1 comprises: a device body 2 comprising a funnel part 20 that decreases in diameter toward a sampling container connection opening 24, and legs 31a, 31b, 31c; and a plate-shaped member 4 housed within the upper part of the funnel part 20. The funnel part 20 comprises a plurality of side holes 26, and the plate-shaped member 4 comprises a plurality of through-holes 42. The device body 2 and plate-shaped member 4 are submerged in liquid nitrogen, in which state a biological-cell-containing liquid is introduced dropwise from above the plate-shaped member 4, thereby forming frozen biological cell pellets in the through-holes 42 of the plate-shaped member 4. The frozen biological cell pellets descend through the funnel part and are guided into the sampling container connection opening 24.
(FR) La présente invention concerne un dispositif de préparation de culot de cellules biologiques congelées 1 qui comprend : un corps de dispositif 2 comprenant une partie entonnoir 20 qui diminue en diamètre vers une ouverture de connexion de récipient d'échantillonnage 24, et des pieds 31a, 31b, 31c ; et un élément en forme de plaque 4 logé à l'intérieur de la partie supérieure de la partie entonnoir 20. La partie entonnoir 20 comprend une pluralité de trous latéraux 26, et l'élément en forme de plaque 4 comprend une pluralité de trous traversants 42. Le corps de dispositif 2 et l'élément en forme de plaque 4 sont immergés dans de l'azote liquide, un liquide contenant des cellules biologiques étant introduit goutte à goutte depuis un emplacement situé au-dessus de l'élément en forme de plaque 4, formant ainsi des culots de cellules biologiques congelées dans les trous traversants 42 de l'élément en forme de plaque 4. Les culots de cellules biologiques congelées descendent à travers la partie entonnoir et sont guidés dans l'ouverture de connexion de récipient d'échantillonnage 24.
(JA) 生体細胞粒状凍結体作成具1は、採取容器接続用開口部24に向かって縮径する漏斗部20と、脚部31a,31b,31cとを備える作成具本体2と、漏斗部20の上部に収納された板状部材4とを備える。漏斗部20は、複数の側孔26を備え、板状部材4は、複数の貫通孔42を備える。作成具本体2および板状部材4は、液体窒素内に沈むものであり、液体窒素に浸漬された状態にて、板状部材4の上方から生体細胞含有液体を滴下することにより、板状部材4の貫通孔42内にて、生体細胞粒状凍結体が形成され、生体細胞粒状凍結体が、漏斗部を下降し、採取容器接続用開口部24に誘導されるものとなっている。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)