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1. (WO2018198682) VIRUS TEST METHOD AND VIRUS TEST KIT

Pub. No.:    WO/2018/198682    International Application No.:    PCT/JP2018/014068
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Tue Apr 03 01:59:59 CEST 2018
IPC: C12Q 1/686
C12N 15/10
C12Q 1/70
Applicants: TOYOBO CO., LTD.
東洋紡株式会社
Inventors: TERAUCHI, Kenta
寺内 謙太
Title: VIRUS TEST METHOD AND VIRUS TEST KIT
Abstract:
Provided are a method and a kit with which it is possible to detect the presence of RNA of a non-enveloped virus by addition to a reaction solution and one-step RT-PCR without isolation of the RNA from a sample or advance heat treatment of the sample. A method for testing whether or not a virus is present, the method comprising the following steps (1) to (3). (1) A step for mixing a sample and a reagent that contains a polar organic solvent. (2) A step for adding a one-step RT-PCR reaction solution that contains reverse transferase and DNA polymerase, or DNA polymerase having reverse transcription activity, to the mixed solution. (3) A step for performing a one-step RT-PCR reaction after sealing the reaction vessel.