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1. (WO2018197533) ANTIBODY SELECTION METHOD
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/197533 International Application No.: PCT/EP2018/060536
Publication Date: 01.11.2018 International Filing Date: 25.04.2018
IPC:
C12P 21/02 (2006.01) ,G01N 33/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
F. HOFFMANN-LA ROCHE AG [CH/CH]; Grenzacherstrasse 124 4070 Basel, CH (AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BE, BF, BG, BH, BJ, BN, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CL, CM, CN, CO, CR, CU, CY, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IR, IS, IT, JO, JP, KE, KG, KH, KM, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, ME, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SI, SK, SL, SM, SN, ST, SV, SY, SZ, TD, TG, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, ZA, ZM, ZW)
HOFFMANN-LA ROCHE INC. [US/US]; Great Notch, 150 Clove Road, 8th Floor Little Falls, New Jersey 07424, US (US)
Inventors:
KETTENBERGER, Hubert; DE
RICHTER, Wolfgang; CH
LARIVIERE, Laurent; DE
KRAFT, Thomas; DE
EMRICH, Thomas; DE
Agent:
SKOLAUT, Alexander; DE
BURGER, Alexander; DE
EISENMANN, Ulrike; DE
GOEHRING, Frank; DE
HILDEBRANDT, Martin; DE
JENNI, Wolfgang; DE
MERTES, Maria; DE
MISERA, Simon; DE
NASER, Werner; DE
WOLTERS, Brit; DE
HEINEMEYER, Thomas; DE
HEYSE, Gero; DE
Priority Data:
17168633.028.04.2017EP
17209268.621.12.2017EP
Title (EN) ANTIBODY SELECTION METHOD
(FR) PROCÉDÉ DE SÉLECTION D'ANTICORPS
Abstract:
(EN) Herein is reported a method for selecting an antibody with a systematic clearance in cynomolgus monkeys of less than 8 m L/kg/day comprising the steps of measuring the retention time of the antibody on performing an FcRn affinity chromatography with a positive linear pH gradient and on a heparin affinity chromatography with a positive linear conductivity/salt gradient, and selecting an antibody that has a relative retention time on the FcRn affinity chromatography column is less than 1.78 times the retention time difference between peaks 2 and 3 the retention time of preparation of an oxidized anti-Her3 antibody of SEQ ID NO: 03 and 04, and a relative retention time on the heparin affinity chromatography column is less than 0.87 times the retention time of an anti-pTau antibody of SEQ ID NO: 01 and 02.
(FR) La présente invention concerne un procédé de sélection d'un anticorps présentant une clairance systématique chez des singes crabiers inférieure à 8 mL/kg/jour comprenant les étapes consistant à mesurer le temps de rétention de l'anticorps lors de la réalisation d'une chromatographie d'affinité avec FcRn avec un gradient de pH linéaire positif et lors d'une chromatographie d'affinité avec l'héparine avec un gradient de conductivité/sel linéaire positif, et à sélectionner un anticorps dont le temps de rétention relatif sur la colonne de chromatographie d'affinité avec FcRn est inférieur à 1,78 fois la différence de temps de rétention entre les pics 2 et 3, le temps de rétention de préparation d'un anticorps anti-Her3 oxydé de SEQ ID N° : 03 et 04, et dont un temps de rétention relatif sur la colonne de chromatographie d'affinité avec l'héparine est inférieur à 0,87 fois le temps de rétention d'un anticorps anti-pTau de SEQ ID N° : 01 et 02.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)