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1. (WO2018197020) GENOME EDITING BY CRISPR-CAS9 USING SHORT DONOR OLIGONUCLEOTIDES

Pub. No.:    WO/2018/197020    International Application No.:    PCT/EP2017/060566
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Thu May 04 01:59:59 CEST 2017
IPC: C12N 15/113
C12N 15/63
Applicants: NOVOZYMES A/S
Inventors: KLITGAARD, Dorte, M.K.
Title: GENOME EDITING BY CRISPR-CAS9 USING SHORT DONOR OLIGONUCLEOTIDES
Abstract:
The present invention relates to methods for modifying at least one target sequence in the genome of a host cell by employing a Class II Cas9 enzyme, e.g., the S. pyogenes Cas9, together with a suitable guide RNA for each target sequence to generate a site-specific cut or nick in at least one genome target sequence, followed by the repair of the cut(s) and/or nick(s) via integration of one or more modified modified donor part of the host cell genome through double recombination on each side of the nick(s).