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1. (WO2018196975) PROBE
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Pub. No.: WO/2018/196975 International Application No.: PCT/EP2017/060043
Publication Date: 01.11.2018 International Filing Date: 27.04.2017
IPC:
C12Q 1/68 ,C12Q 1/70 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
Applicants:
THE UNIVERSITY OF SHEFFIELD [GB/GB]; Firth Court Western Bank Sheffield South Yorkshire S10 2TN, GB
Inventors:
EL-KHAMISY, Sherif; GB
SHAWKY, Sherif; EG
Agent:
THURSTON, Joanna; GB
Priority Data:
Title (EN) PROBE
(FR) SONDE
Abstract:
(EN) A capture ligand for an analyte comprising SEQ ID NO:1, or comprising an antisense primer of at least 25 bases complementary to SEQ ID NO:2, wherein the primer initiates hybridisation from any of nucleotides 5 to 20, 43 to 118, 156 to 238, 253 to 279 or 291 to 314 of SEQ ID NO:2. A probe for an analyte comprising this capture ligand and a probe comprising a metal nanoparticle and one or more capture ligand specifically for the indicator of disease conjugated thereto. Also assay kits and methods of detecting the presence of an analyte.
(FR) La présente invention concerne un ligand de capture pour analyte comprenant SEQ ID no : 1, ou comprenant une amorce antisens d’au moins 25 bases complémentaires de SEQ ID no : 2, où l’amorce initie l’hybridation à partir de n’importe lesquels des nucléotides 5 à 20, 43 à 118, 156 à 238, 253 à 279 ou 291 à 314 de SEQ ID no : 2. Une sonde pour un analyte comprenant ce ligand de capture et une sonde comprenant une nanoparticule métallique et un ou plusieurs ligands de capture spécifiquement pour l’indicateur de maladie conjugué à cette dernière. La présente invention concerne également des kits et des procédés d’essai de détection de la présence d’un analyte.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)