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1. (WO2018196932) A METHOD AND A DEVICE FOR QUANTIFYING LIVING ORGANISMS AND USE OF A DEVICE
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Pub. No.: WO/2018/196932 International Application No.: PCT/DK2017/050131
Publication Date: 01.11.2018 International Filing Date: 28.04.2017
IPC:
G01N 21/64 (2006.01) ,G01N 33/18 (2006.01) ,G01N 15/14 (2006.01) ,C12Q 1/02 (2006.01) ,C12Q 1/06 (2006.01) ,C02F 103/00 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
18
Water
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10
Investigating individual particles
14
Electro-optical investigation
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06
Quantitative determination
C CHEMISTRY; METALLURGY
02
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
F
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
103
Nature of the water, waste water, sewage or sludge to be treated
Applicants:
MICROWISE APS [DK/DK]; Munkegårdsvej 6 B 3490 Kvistgård, DK
Inventors:
BLACKBURN, Nicholas David; DK
POULSEN, Per-Ove; DK
ENGELL-SØRENSEN, Kirsten; DK
HAECKY, Pia Bodil; DK
Agent:
PATENTGRUPPEN A/S; Aaboulevarden 31, 4th floor DK-8000 Aarhus C, DK
Priority Data:
Title (EN) A METHOD AND A DEVICE FOR QUANTIFYING LIVING ORGANISMS AND USE OF A DEVICE
(FR) PROCÉDÉ ET DISPOSITIF DE QUANTIFICATION D'ORGANISMES VIVANTS ET UTILISATION D'UN DISPOSITIF
Abstract:
(EN) Disclosed is a method for quantifying living organisms (1, 4) in a liquid sample (2), the method comprising the steps of • guiding the liquid sample (2) into a chamber (3), • analysing pictures of the sample (2) inside the chamber (3) to detect the number of organisms (4) moving by themselves in the sample, • illuminating the sample (2) with light in at least a part of the violet-blue spectrum while detecting the number of organisms (1) that are fluorescent in the sample inside the chamber (3), and • analysing pictures of the sample (2) inside the chamber (3) while illuminating the sample (2) with light in at least a part of the violet-blue spectrum to detect the number of organisms (1, 4) that are both moving by themselves and fluorescent. A device (15) for quantifying living organisms (1, 4) and use of a device (15) is also disclosed.
(FR) L'invention concerne un procédé de quantification d'organismes vivants (1, 4) dans un échantillon liquide (2), le procédé comprenant les étapes suivantes : • guidage de l'échantillon liquide (2) dans une chambre (3), • analyse des images de l'échantillon (2) à l'intérieur de la chambre (3) pour détecter le nombre d'organismes (4) se déplaçant par eux-mêmes dans l'échantillon, • éclairage de l'échantillon (2) avec de la lumière dans au moins une partie du spectre violet-bleu tout en détectant le nombre d'organismes (1) qui sont fluorescents dans l'échantillon à l'intérieur de la chambre (3), et • analyse d'images de l'échantillon (2) à l'intérieur de la chambre (3) tout en éclairant l'échantillon (2) avec de la lumière dans au moins une partie du spectre violet-bleu pour détecter le nombre d'organismes (1, 4) qui à la fois se déplacent par eux-mêmes et sont fluorescents. L'invention concerne également un dispositif (15) de quantification des organismes vivants (1, 4) ainsi que l'utilisation d'un dispositif (15).
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)