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1. (WO2018196691) PRECISE RECOGNITION METHOD FOR NUCLEIC ACID

Pub. No.:    WO/2018/196691    International Application No.:    PCT/CN2018/083921
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Sat Apr 21 01:59:59 CEST 2018
IPC: C12Q 1/68
G01N 21/64
Applicants: NI, Yanxiang
倪燕翔
Inventors: NI, Yanxiang
倪燕翔
NIU, Gang
牛钢
MA, Tszshan
马紫珊
CAO, Bo
曹博
ZHANG, Michael Qiwei
张奇伟
Title: PRECISE RECOGNITION METHOD FOR NUCLEIC ACID
Abstract:
Provided is a recognition method for a nucleic acid. The recognition method comprises the following steps: a. making contact with a sample with a hybridization probe; b. optionally eluting the hybridization probe that is not bound to a target nucleic acid; and c. recognizing whether the hybridization probe is bound to the target nucleic acid, wherein the hybridization probe comprises reporter groups, and the hybridization probe, so that recognizable signals are produced by the reporter groups, has different states under the two following conditions: (i) the hybridization probe is in a free state, and (ii) the hybridization probe is bound to the target nucleic acid. Also provided is the use of the hybridization probe in the fluorescence microscopy imaging of the target nucleic acid. The recognition method can simplify the operation steps for recognizing the nucleic acid, efficiently mark the target nucleic acid molecules, decrease the non-specific binding of the probe to nucleic acids other than the target nucleic acid, improve the recognition efficiency and accuracy, and particularly benefit recognition for nucleic acids with smaller length and/or non-repetitive sequences, and is suitable for acquiring super-resolution images of the target nucleic acid.