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1. (WO2018196344) METHOD FOR CULTURING PANCREATIC CANCER CELLS UNDER HYPOXIC ENVIRONMENT

Pub. No.:    WO/2018/196344    International Application No.:    PCT/CN2017/111192
Publication Date: Fri Nov 02 00:59:59 CET 2018 International Filing Date: Thu Nov 16 00:59:59 CET 2017
IPC: C12N 5/09
C12N 5/095
Applicants: SUN, Chengyi
孙诚谊
Inventors: SUN, Chengyi
孙诚谊
ZHANG, Hong
张宏
PAN, Yaozhen
潘耀振
YU, Chao
喻超
TIAN, She
田舍
HE, Zhiwei
何志伟
CHEN, Ling
陈玲
Title: METHOD FOR CULTURING PANCREATIC CANCER CELLS UNDER HYPOXIC ENVIRONMENT
Abstract:
A method for culturing pancreatic cancer cells under a hypoxic environment, comprising culturing pancreatic cancer cells AsPC-1, BxPC-3, or PANC-1 under a hypoxic condition with a volume ratio of 1.5:5:93.5 for O2, CO2, and N2. The AsPC-1 or BxPC-3 is cultured in a rpmi-1640 medium containing 10% FBS, and the PANC-1 is cultured in a DMEM medium containing 10% FBS.