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1. (WO2018189124) METHOD FOR OBTAINING DIFFERENTIATED CELLS FROM MUSCLE DERIVED PROGENITOR CELLS
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Claims

1. A method for preparing differentiated mammalian cells, comprising (i) collecting a

muscle microbiopsy sample from a mammalian individual, (ii) placing the collected muscle sample of step (i) in a suitable culture medium, (iii) growing the cells emerging from said microbiopsy, (iv) separating the microbiopsy and growing cells when a sufficient amount of growing cells is present around the muscle sample, (v) continuing the growing of the cells to near confluency, and (vi) differentiating the cells obtained at (v) in a suitable differentiation medium, in the absence of any separation technique intended to isolate certain mesenchymal stem cell populations.

2. Method according to claim 1 wherein the said muscle microbiopsy is taken from skeletal muscle tissue.

3. Method according to claim 1 or 2 wherein the micro-biopsy contains about 10 to about 30 mg, preferably more than about 12 mg, more preferably more than about 15 mg and/or less than about 25 mg, preferably less than about 20 mg.

4. Method according to any preceding claim wherein differentiation is made into

adipocytes, osteocytes, chondrocytes, myogenic cells, hematopoetic cells, endothelial cells, neural cells, cardiac cells, or hepatocytes by culturing them in an adequate adipogenic, osteogenic, chondrogenic, myogenic, hematopoetic, endothelial, neuronal, cardial, or hepatocytic differentiation medium, respectively.

5. Differentiated adipocytes, osteocytes, chondrocytes, myogenic cells, hematopoetic cells, endothelial cells, neural cells, cardiac cells, or hepatocytes as obtained by the method of any of cairns 1 to 4.

6. A pharmaceutical or veterinary composition comprising differentiated adipocytes,

osteocytes, chondrocytes, myogenic cells, hematopoetic cells, endothelial cells, neural cells, cardiac cells, or hepatocytes of claim 5.

7. A substrate comprising differentiated cells of claim 5 on part at least of its surface.

8. A method of treatment of mammalian subjects comprising administering differentiated cells of claim 5 to subjects in need thereof.

9. The method of treatment of claim 8 comprising the treatment of one or more of the following disorders: desmitis, osteochondrosis, arthritis, osteoporosis, tendonitis, laminitis, inflammation of the tendons and ligaments, fracture, and failure to heal in a mammalian subject.