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1. (WO2018189121) NEW USES OF MAMMALIAN MUSCLE-DERIVED STEM CELLS
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Claims

1 . A method for assessing the efficacy and/or adverse effects of a compound or

composition, preferably a pharmaceutical compound or composition, on a mammal, comprising obtaining mammalian pluripotent muscle-derived mesenchymal stem cells or a population thereof, exposing the said stem cells or stem cell population to the relevant compound or composition and assessing the effects of the relevant compound or composition on the said stem cells or stem cell population, wherein the mammalian pluripotent muscle-derived stem cells are obtained by muscular micro-biopsy, followed by density centrifugation, and are positive for at least CD105 and negative for CD45, MHCII and CD29.

2. Method according to claim 1 wherein the mammalian pluripotent muscle-derived stem cells are positive for CD44, CD90 and CD105 and negative for CD45, MHCII and CD29.

3. Method according to claim 1 or 2 wherein the mammalian pluripotent muscle-derived stem cells further are positive for mi'R-128, mi'R-133B, and miR-802.

4. Method according to claim 3 wherein the mammalian pluripotent muscle-derived stem cells further are slightly positive for mi'R-218 and negative for mi'R-656.

5. Method according to any preceding claim wherein the effects of the pharmaceutical compound or composition are assessed by comparison with a control conducted in the absence of the relevant compound or composition.

6. Method according to any of claims 1 to 5 wherein the method is a drug screening

method and the pharmaceutical compound or composition is tested on the said stem cells or stem cell population derived from a multitude of individuals, or on

differentiated cells derived therefrom.

7. Method according to any of claims 1 to 5 wherein the said mammalian pluripotent muscle-derived stem cells or a population thereof are derived from a mammalian patient, the method further comprising assessing the likelihood of a positive response by the mammalian patient by evaluating the level of a positive response of the said mammalian pluripotent muscle-derived stem cells or a population thereof or differentiated cells derived therefrom.