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1. (WO2018184086) OLIGONUCLEOTIDES, SET OF OLIGONUCLEOTIDES, AND METHOD FOR SIMULTANEOUSLY DETECTING MAYV, OROV AND OROV-LIKE, KIT FOR DIAGNOSING AND DISCRIMINATING MAYV, OROV AND OROV-LIKE INFECTIONS

Pub. No.:    WO/2018/184086    International Application No.:    PCT/BR2018/050092
Publication Date: Fri Oct 12 01:59:59 CEST 2018 International Filing Date: Sat Apr 07 01:59:59 CEST 2018
IPC: C12Q 1/6888
C12Q 1/686
Applicants: FUNDAÇÃO OSWALDO CRUZ
Inventors: NAVECA, Felipe, Gomes
DO NASCIMENTO, Valdinete, Alves
Title: OLIGONUCLEOTIDES, SET OF OLIGONUCLEOTIDES, AND METHOD FOR SIMULTANEOUSLY DETECTING MAYV, OROV AND OROV-LIKE, KIT FOR DIAGNOSING AND DISCRIMINATING MAYV, OROV AND OROV-LIKE INFECTIONS
Abstract:
The present invention provides a real-time multiplex RT-qPCR method which allows, in a single step, simultaneous detection of the Oropouche virus (OROV), Oropouche-like virus and/or Mayaro virus. To this end, primers were developed that are used to amplify particular regions of the genomes of the viruses. The presence of the viruses in a sample is indicated by the formation of fluorescence released by the specific probes for each virus.