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1. (WO2018183706) MATERIALS AND METHODS FOR ALKENE REDUCTION OF LEVOGLUCOSENONE BY AN ALKENE REDUCTASE

Pub. No.:    WO/2018/183706    International Application No.:    PCT/US2018/025184
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Fri Mar 30 01:59:59 CEST 2018
IPC: C12P 17/18
C12P 17/04
C12N 9/02
Applicants: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
AGROPARISTECH
Inventors: STEWART, Jon Dale
ALLAIS, Florent
MOUTERDE, Louis Michel Marie
Title: MATERIALS AND METHODS FOR ALKENE REDUCTION OF LEVOGLUCOSENONE BY AN ALKENE REDUCTASE
Abstract:
The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (,S)-Y-hydroxymethyl-a,P-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 from Pichia stipites and comprises the amino acid sequence of SEQ ID NO: 1. In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).