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1. (WO2018183610) SYSTEMS, ARTICLES, AND METHODS FOR FLOWING PARTICLES

Pub. No.:    WO/2018/183610    International Application No.:    PCT/US2018/025040
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Fri Mar 30 01:59:59 CEST 2018
IPC: C12M 1/34
G01N 15/10
Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventors: KIMMERLING, Robert J.
CALISTRI, Nicholas L.
MANALIS, Scott R.
OLCUM, Selim
STEVENS, Mark M.
Title: SYSTEMS, ARTICLES, AND METHODS FOR FLOWING PARTICLES
Abstract:
Systems and methods for flowing particles, such as biological entities, in a fluidic channel(s) are generally provided. In some cases, the systems described herein are designed such that a single particle may be isolated from a plurality of particles and flowed into a fluidic channel (e.g., a microfluidic channel) and/or collected e.g., on fluidically isolated surfaces. For example, the single particle may be present in a plurality of particles of relatively high density and the single particle is flowed into a fluidic channel, such that it is separated from the plurality of particles. The particles may be spaced within a fluidic channel so that individual particles may be measured/observed over time. In certain embodiments, the particle may be a biological entity. Such article and methods may be useful, for example, for isolating single cells into individual wells of multi-well cell culture dishes (e.g., for single-cell analysis).