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1. (WO2018182555) MICROORGANISMS WHICH ARE EFFECTIVE FOR PREVENTING PLANT'S COLD STRESS

Pub. No.:    WO/2018/182555    International Application No.:    PCT/TR2017/050480
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Fri Oct 06 01:59:59 CEST 2017
IPC: A01N 63/00
A01N 63/02
C12N 1/20
C12R 1/01
Applicants: YEDİTEPE SAĞLIK HİZMETLERİ ANONİM ŞİRKETİ
Inventors: TURAN, Metin
Title: MICROORGANISMS WHICH ARE EFFECTIVE FOR PREVENTING PLANT'S COLD STRESS
Abstract:
The present invention is directed to a microbial inoculant that is effective for preventing cold stress. In an important aspect of the invention, the microbial inoculant includes the Azospirillum brasilense, Bacillus subtilis, Bacillus megaterium, Lactocoecus spp., Pseudomonas sp, Pseudomonas putita, Pseudomonas lurida, Acinetobacter, Paenibacillus sp, Burkholderia phytofirmans. The use of those as a microbial inoculant is effective for increasing the productivity of both nonlegume and legume plants as well as vegetable plants over a wide variety of soil types and climates. One important aspect of the invention is directed to biologically pure cultures of Azospirillum brasilense, Bacillus subtilis, Bacillus megaterium, Lactocoecus spp., Pseudomonas sp, Pseudomonas putita, Pseudomonas lurida, Acinetobacter, Paenibacillus sp, Burkholderia phytofirmans. Azospirillum brasilense, Bacillus subtilis, Bacillus megaterium, Lactocoecus spp., Pseudomonas sp, Pseudomonas putita, Pseudomonas lurida, Acinetobacter, Paenibacillus sp, Burkholderia phytofirmans, may be used as a microbial inoculant either alone or in combination with other agronomically beneficial microorganisms. Application of the microbial inoculant to the plant or the soil prevent cold stress, provides an increased level of soil nitrogen, improves mineral uptake into plants, stimulates plant growth through the production of plant growth regulators, and inhibits phytopathogenic microflora. In another important aspect, the present invention provides a method for growing Azospirillum brasilense, Bacillus subtilis, Bacillus megaterium, Lactocoecus spp., Pseudomonas sp, Pseudomonas putita, Pseudomonas lurida, Acinetobacter, Paenibacillus sp, Burkholderia phytofirmans. In accordance with the method of the invention, Azospirillum brasilense, Bacillus subtilis, Bacillus megaterium, Lactocoecus spp., Pseudomonas sp, Pseudomonas putita, Pseudomonas lurida, Acinetobacter, Paenibacillus sp, Burkholderia phytofirmans is inoculated into a plant extract medium and cells are growth under conditions effective for providing a cell density of about 108 to about 109 cfu/ml.