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|1. (WO2018182044) CELL CULTURE SUBSTRATE, CANCER CELL AGGREGATE AND METHOD FOR MANUFACTURING SAME USING SAID SUBSTRATE, AND DRUG SCREENING METHOD USING SAID CANCER CELL AGGREGATE|
|Applicants:||NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
|Title:||CELL CULTURE SUBSTRATE, CANCER CELL AGGREGATE AND METHOD FOR MANUFACTURING SAME USING SAID SUBSTRATE, AND DRUG SCREENING METHOD USING SAID CANCER CELL AGGREGATE|
[Problem] An objective of the present invention is the in-vitro production of a cancer cell population retaining native biological properties, such as morphological polarity and tissue motion polarity, of the original cancer tissue. [Solution] The present invention pertains to a cell culture substrate comprising a base material and a biocompatible polymer layer, the cell culture substrate having, at prescribed intervals on the substrate surface, a plurality of rough sections having a prescribed surface structure having a prescribed shape and not covered by the biocompatibile polymer layer. The present invention makes it possible to use a very simple process, namely culturing cancer cells on a cell culture substrate having a prescribed structure, to obtain, in a living state, a cancer cell aggregate having the same morphological polarity and tissue motion polarity as is observed in-vivo, whereby the heretofore impossible live imaging of micro tumors in-vitro is made possible. Since it is considered that said cancer cell aggregate would reproduce the in-vivo sequence of cancer development, proliferation, invasion, metastasis and recurrence, the cancer cell aggregate could be used as a research tool for cancer research or for screening cancer drugs.