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1. (WO2018182044) CELL CULTURE SUBSTRATE, CANCER CELL AGGREGATE AND METHOD FOR MANUFACTURING SAME USING SAID SUBSTRATE, AND DRUG SCREENING METHOD USING SAID CANCER CELL AGGREGATE

Pub. No.:    WO/2018/182044    International Application No.:    PCT/JP2018/014119
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Tue Apr 03 01:59:59 CEST 2018
IPC: C12M 1/00
C12N 5/09
C12Q 1/02
G01N 33/15
G01N 33/50
Applicants: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
国立大学法人北海道大学
Inventors: MIYATAKE Yukiko
宮武 由甲子
SHIGETOMI Kaori
繁富 香織
OKAJIMA Takaharu
岡嶋 孝治
KASAHARA Masanori
笠原 正典
Title: CELL CULTURE SUBSTRATE, CANCER CELL AGGREGATE AND METHOD FOR MANUFACTURING SAME USING SAID SUBSTRATE, AND DRUG SCREENING METHOD USING SAID CANCER CELL AGGREGATE
Abstract:
[Problem] An objective of the present invention is the in-vitro production of a cancer cell population retaining native biological properties, such as morphological polarity and tissue motion polarity, of the original cancer tissue. [Solution] The present invention pertains to a cell culture substrate comprising a base material and a biocompatible polymer layer, the cell culture substrate having, at prescribed intervals on the substrate surface, a plurality of rough sections having a prescribed surface structure having a prescribed shape and not covered by the biocompatibile polymer layer. The present invention makes it possible to use a very simple process, namely culturing cancer cells on a cell culture substrate having a prescribed structure, to obtain, in a living state, a cancer cell aggregate having the same morphological polarity and tissue motion polarity as is observed in-vivo, whereby the heretofore impossible live imaging of micro tumors in-vitro is made possible. Since it is considered that said cancer cell aggregate would reproduce the in-vivo sequence of cancer development, proliferation, invasion, metastasis and recurrence, the cancer cell aggregate could be used as a research tool for cancer research or for screening cancer drugs.