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1. (WO2018182016) CELL CULTURING USING NANOFIBERS

Pub. No.:    WO/2018/182016    International Application No.:    PCT/JP2018/013977
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Sat Mar 31 01:59:59 CEST 2018
IPC: C12M 3/00
A61K 35/28
A61L 27/20
A61L 27/38
A61L 27/40
A61L 27/50
A61L 27/58
A61P 43/00
C12N 5/071
Applicants: NISSAN CHEMICAL CORPORATION
日産化学株式会社
Inventors: KANAKI, Tatsuro
金木 達朗
KIDA, Katsuhiko
木田 克彦
HAYASHI, Hisato
林 寿人
MINAMI, Masataka
南 昌孝
Title: CELL CULTURING USING NANOFIBERS
Abstract:
The present invention provides: a cell carrier that contains nanofibers composed of a water-insoluble polysaccharide, preferably chitin or chitosan nanofibers, the carrier is possibly used as a common carrier for various operations including i) suspension culturing, ii) differentiation induction, iii) transport and storage under non-freezing conditions, and iv) transplantation of adhesive cells, and v) recovery of a physiologically active substance from a culture supernatant; and continuous execution of, using said carrier, a plurality of operations selected from among i) suspension culturing, ii) differentiation induction, iii) transport and storage under non-freezing conditions, and iv) transplantation of adhesive cells, and v) recovery of a physiologically active substance from a culture supernatant.