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1. (WO2018181997) METHOD FOR DETECTING CARBAPENEMASE-PRODUCING BACTERIUM
Latest bibliographic data on file with the International BureauSubmit observation

Pub. No.: WO/2018/181997 International Application No.: PCT/JP2018/013920
Publication Date: 04.10.2018 International Filing Date: 30.03.2018
IPC:
C12Q 1/04 (2006.01) ,C12M 1/34 (2006.01) ,C12Q 1/34 (2006.01) ,G01N 33/15 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/52 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
15
Medicinal preparations
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52
Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
Applicants:
国立大学法人大阪大学 OSAKA UNIVERSITY [JP/JP]; 大阪府吹田市山田丘1番1号 1-1, Yamadaoka, Suita-shi, Osaka 5650871, JP
Inventors:
竹内 壇 TAKEUCHI, Dan; JP
浜田 茂幸 HAMADA, Shigeyuki; JP
朝野 和典 TOMONO, Kazunori; JP
明田 幸宏 AKEDA, Yukihiro; JP
Agent:
岩谷 龍 IWATANI, Ryo; JP
Priority Data:
2017-07305631.03.2017JP
Title (EN) METHOD FOR DETECTING CARBAPENEMASE-PRODUCING BACTERIUM
(FR) PROCÉDÉ POUR LA DÉTECTION D'UNE BACTÉRIE PRODUISANT DE LA CARBAPÉNÉMASE
(JA) カルバペネマーゼ産生菌の検出方法
Abstract:
(EN) A method for detecting a carbapenemase-producing bacterium, comprising: step 1 which is a step of adding an antibacterial drug to a sample and culturing the sample; step 2 which is a step of measuring an absorbance (A-Abs) of a culture obtained in step 1 at a maximum wavelength (Anm) of a maximum absorption of the antibacterial drug and an absorbance (B-Abs) of the culture at a wavelength (Bnm) that is longer by at least 40 nm than the wavelength (Anm); step 3 which is a step of assigning the absorbance (B-Abs) obtained in step 2 to a calibration curve to calculate a blank absorbance (C-Abs); and step 4 which is a step of determining that a carbapenemase-producing bacterium is contained in the sample when the difference between the absorbance (A-Abs) obtained in step 2 and the absorbance (C-Abs) obtained in step 3 is smaller than a predetermined threshold value. According to the detection method of the present invention, a carbapenemase-producing bacterium can be detected conveniently and rapidly and with high sensitivity and high specificity. Therefore, the detection method is useful in clinical practices and as a measure against hospital-acquired infection.
(FR) L'invention concerne un procédé de détection d'une bactérie productrice de carbapénémase, comprenant : une étape 1 qui est une étape d'addition d'un médicament antibactérien à un échantillon et de culture de l'échantillon ; une étape 2 qui est une étape de mesure d'une absorbance (A-Abs) d'une culture obtenue à l'étape 1 à une longueur d'onde maximale (Anm) d'une absorption maximale du médicament antibactérien et d'une absorbance (B-Abs) de la culture à une longueur d'onde (Bnm) qui est plus longue d'au moins 40 nm que la longueur d'onde (Anm) ; une étape 3 qui est une étape d'attribution de l'absorbance (B-Abs) obtenue à l'étape 2 à une courbe d'étalonnage pour calculer une absorbance à blanc (C-Abs) ; et une étape 4 qui est une étape de détermination du fait qu'une bactérie productrice de carbapénémase est contenue dans l'échantillon lorsque la différence entre l'absorbance (A-Abs) obtenue à l'étape 2 et l'absorbance (C-Abs) obtenue à l'étape 3 est inférieure à une valeur seuil prédéterminée. Selon le procédé de détection de la présente invention, une bactérie productrice de carbapénémase peut être détectée de manière commode et rapide et avec une sensibilité élevée et une spécificité élevée. Par conséquent, le procédé de détection est utile dans des pratiques cliniques et en tant que mesure contre une infection acquise à l'hôpital.
(JA) 工程1:試料に抗菌薬を添加して培養する工程、工程2:工程1で得られた培養液について、前記抗菌薬の最大吸収の極大波長(Anm)における吸光度(A-Abs)と、該波長から少なくとも40nm高波長側の波長(Bnm)における吸光度(B-Abs)を測定する工程、工程3:工程2で得られた吸光度(B-Abs)を検量線に当てはめブランク吸光度(C-Abs)を算出する工程、及び工程4:工程2で得られた吸光度(A-Abs)と工程3で得られた吸光度(C-Abs)の差が予め設定された閾値を下回る場合は試料にカルバペネマーゼ産生菌が含まれると判定する工程、を含むカルバペネマーゼ産生菌の検出方法。本発明の検出方法により、高い感度・特異度で簡便かつ迅速にカルバペネマーゼ産生菌を検出することができるため、臨床及び院内感染対策上有用である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)