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1. (WO2018181960) METHOD FOR PRODUCING OSTEOBLAST CLUSTER USING HUMAN iPS CELLS

Pub. No.:    WO/2018/181960    International Application No.:    PCT/JP2018/013777
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Sat Mar 31 01:59:59 CEST 2018
IPC: C12N 5/077
A61L 27/36
A61L 27/38
C12N 5/074
Applicants: TOHOKU UNIVERSITY
国立大学法人東北大学
Inventors: EGUSA, Hiroshi
江草 宏
OKAWA, Hiroko
大川 博子
Title: METHOD FOR PRODUCING OSTEOBLAST CLUSTER USING HUMAN iPS CELLS
Abstract:
The present invention relates to a method for producing an osteoblast cluster from human iPS cells, the method comprising: (1) a step for inducing the formation of embryoid bodies by non-adhesively culturing undifferentiated human iPS cells; (2) a step for inducing the differentiation of human iPS cells into mesodermal cells by non-adhesively culturing the embryoid bodies of the human iPS cells obtained in step (1); and (3) a step for inducing differentiation into osteoblasts by non-adhesively culturing the mesodermal cells of the human iPS cells obtained in step (2).