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1. (WO2018178069) PERFUSION MEDIUM

Pub. No.:    WO/2018/178069    International Application No.:    PCT/EP2018/057757
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Wed Mar 28 01:59:59 CEST 2018
IPC: C12P 21/02
Applicants: BOEHRINGER INGELHEIM INTERNATIONAL GMBH
Inventors: WANG, Samantha
LIN, Henry
Title: PERFUSION MEDIUM
Abstract:
The invention relates to a method of culturing mammalian cells expressing a heterologous protein in a perfusion cell culture comprising increasing the potassium concentration and decreasing the molar ratio of sodium to potassium to reduce wasteful cell bleed and to increase protein production. The invention further relates to a serum-free perfusion medium comprising a high potassium ion concentration and a low molar ratio of sodium to potassium and to the use of this medium for use in culturing cells in a perfusion culture during production phase or for reducing the cell bleed volume during production phase.