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1. (WO2018178063) PERFUSION MEDIUM

Pub. No.:    WO/2018/178063    International Application No.:    PCT/EP2018/057749
Publication Date: Fri Oct 05 01:59:59 CEST 2018 International Filing Date: Wed Mar 28 01:59:59 CEST 2018
IPC: C12P 21/00
C12P 21/08
Applicants: BOEHRINGER INGELHEIM INTERNATIONAL GMBH
Inventors: OGAWA, Daisie
LIN, Henry
Title: PERFUSION MEDIUM
Abstract:
The invention relates to a method of culturing mammalian cells expressing a heterologous protein in a perfusion cell culture comprising adding iron and a retinoid to reduce wasteful cell bleed during production phase. The invention further relates to a serum-free perfusion medium comprising iron and a retinoid and its use for culturing cells in a perfusion culture during production phase or for reducing the cell bleed volume during production phase.