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1. (WO2018165881) CEPHALOSPORIN C ACYLASE MUTANTS AND APPLICATIONS THEREOF

Pub. No.:    WO/2018/165881    International Application No.:    PCT/CN2017/076688
Publication Date: Fri Sep 21 01:59:59 CEST 2018 International Filing Date: Thu Mar 16 00:59:59 CET 2017
IPC: C12N 9/80
C12N 15/55
C12N 1/21
C12P 35/02
C12R 1/125
C12R 1/865
C12R 1/84
C12R 1/19
Applicants: SHANGHAI GENEWELL BIOTECHNOLOGY CO., LTD
上海星维生物技术有限公司
SHANGHAI RESEARCH AND DEVELOPMENT CENTER OF INDUSTRIAL BIOTECHNOLOGY
上海工业生物技术研发中心
Inventors: YANG, Sheng
杨晟
WANG, Jingang
王金刚
JIANG, Yu
蒋宇
CHEN, Shuming
陈舒明
LIANG, Yan
梁岩
Title: CEPHALOSPORIN C ACYLASE MUTANTS AND APPLICATIONS THEREOF
Abstract:
Provided are cephalosporin C acylase mutants constructed by means of a point mutation method. The cephalosporin C acylase mutants have an amino acid sequence of SEQ ID NOs: 3-12. Compared with wild-type cephalosporin C acylase, the specificity and the enzyme activity of substrates of the mutants are improved, and the mutants can be used for catalyzing the production of 7-aminocephalosporanic acid by using cephalosporin C as a substrate.