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1. (WO2018165629) CYTOSINE TO GUANINE BASE EDITOR

Pub. No.:    WO/2018/165629    International Application No.:    PCT/US2018/021878
Publication Date: Fri Sep 14 01:59:59 CEST 2018 International Filing Date: Sat Mar 10 00:59:59 CET 2018
IPC: C07K 19/00
C12N 9/24
C12N 9/22
C12N 15/62
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventors: LIU, David, R.
KOBLAN, Luke, W.
Title: CYTOSINE TO GUANINE BASE EDITOR
Abstract:
Some aspects of this disclosure provide compositions, strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins capable of inducing a cytosine (C) to guanine (G) change in a nucleic acid (e.g., genomic DNA) are provided. In some embodiments, fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9) and nucleic acid editing proteins or protein domains, e.g., deaminase domains, polymerase domains, and/or base excision enzymes are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9), and nucleic acid editing proteins or domains, are provided.