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1. (WO2018163947) PROTEIN CRYSTALLIZATION UTILIZING POROUS PROTEIN CRYSTAL
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/163947 International Application No.: PCT/JP2018/007697
Publication Date: 13.09.2018 International Filing Date: 01.03.2018
IPC:
C12N 15/09 (2006.01) ,C07K 1/14 (2006.01) ,C07K 19/00 (2006.01) ,C12N 1/15 (2006.01) ,C12N 1/19 (2006.01) ,C12N 1/21 (2006.01) ,C12N 5/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
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Extraction; Separation; Purification
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
19
Hybrid peptides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
15
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
19
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
国立大学法人徳島大学 TOKUSHIMA UNIVERSITY [JP/JP]; 徳島県徳島市新蔵町2丁目24番地 2-24, Shinkura-cho, Tokushima-shi, Tokushima 7708501, JP
Inventors:
真板 宣夫 MAITA, Nobuo; JP
Agent:
特許業務法人三枝国際特許事務所 SAEGUSA & PARTNERS; 大阪府大阪市中央区道修町1-7-1 北浜TNKビル Kitahama TNK Building, 1-7-1, Doshomachi, Chuo-ku, Osaka-shi, Osaka 5410045, JP
Priority Data:
2017-04245607.03.2017JP
Title (EN) PROTEIN CRYSTALLIZATION UTILIZING POROUS PROTEIN CRYSTAL
(FR) CRISTALLISATION DE PROTÉINE À L'AIDE D'UN CRISTAL POREUX PROTÉINIQUE
(JA) 多孔性タンパク質結晶を利用したタンパク質結晶化
Abstract:
(EN) Disclosed are: a fusion protein containing an R1-EN and a protein fused to a C-terminus or an N-terminus of said R1-EN or a protein having said R1-EN in a loop; a protein crystal production method including a step for crystallizing said fusion protein; protein crystals containing said fusion protein; a protein structure analyzing method including a step for performing X-ray crystal structure analysis by using the protein crystals obtained by means of the aforementioned method or the aforementioned protein crystals; a nucleic acid coding for said fusion protein; an expression vector containing said nucleic acid; a transformant in which said nucleic acid has been introduced; and a method for placing an arbitrary protein in a gap of a crystal lattice having a honeycomb structure in which said fusion protein is used.
(FR) L'invention concerne : une protéine de fusion contenant un R1-EN et une protéine fusionnée à une extrémité C-terminale ou à une extrémité N-terminale dudit R1-EN ou d'une protéine présentant ledit R1-EN dans une boucle ; un procédé de production de cristal protéinique comprenant une étape de cristallisation de ladite protéine de fusion ; des cristaux protéiniques contenant ladite protéine de fusion ; un procédé d'analyse de structure protéinique comprenant une étape consistant à effectuer une analyse de structure de cristal par rayons X à l'aide des cristaux protéiniques obtenus au moyen du procédé susmentionné ou des cristaux protéiniques susmentionnés ; un acide nucléique codant pour ladite protéine de fusion ; un vecteur d'expression contenant ledit acide nucléique ; un transformant dans lequel ledit acide nucléique a été introduit ; et un procédé consistant à placer une protéine arbitraire dans un interstice d'un réseau cristallin présentant une structure en nid d'abeilles dans laquelle ladite protéine de fusion est utilisée.
(JA) 開示されているのは、R1-EN、及び該R1-ENのC末端若しくはN末端に融合したタンパク質又はループ中に該R1-ENを有するタンパク質を含有する融合タンパク質、該融合タンパク質を結晶化させる工程を含むタンパク質結晶の製造方法、該融合タンパク質を含むタンパク質結晶、該方法により得られるタンパク質結晶又は該タンパク質結晶を用いてX線結晶構造解析を行う工程を含むタンパク質の構造解析方法、該融合タンパク質をコードする核酸、該核酸を含む発現ベクター、該核酸が導入された形質転換体、並びに該融合タンパク質を使用するハニカム構造をした結晶格子の空隙に任意のタンパク質を配置させる方法である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)