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1. WO2018161177 - NUCLEIC ACID ENZYME-MEDIATED SIGNAL AMPLIFICATION FOR BIOSENSING

Publication Number WO/2018/161177
Publication Date 13.09.2018
International Application No. PCT/CA2018/050294
International Filing Date 12.03.2018
IPC
C12Q 1/6844 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/68 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12Q 1/682 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
682Signal amplification
CPC
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
C12Q 1/682
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
682Signal amplification
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/689
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
689for bacteria
C12Q 2600/178
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
178miRNA, siRNA or ncRNA
Applicants
  • MCMASTER UNIVERSITY [CA]/[CA]
Inventors
  • LI, Yingfu
  • BRENNAN, John
  • LIU, Meng
Agents
  • BERESKIN & PARR LLP/S.E.N.C.R.L., S.R.L.
Priority Data
62/469,77210.03.2017US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NUCLEIC ACID ENZYME-MEDIATED SIGNAL AMPLIFICATION FOR BIOSENSING
(FR) AMPLIFICATION DE SIGNAL, MÉDIÉE PAR UNE ENZYME, D'ACIDE NUCLÉIQUE POUR BIODÉTECTION
Abstract
(EN)
Described is an approach that takes advantage of rolling circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD) to achieve massive signal amplification for biosensing via a cross-feedback mechanism. An RCA reaction generates copies of an RCD that triggers a reaction cascade designed to generate additional DNA assemblies for RCA. These cross- feedback actions work autonomously to turn limited molecular recognition events into massive amounts of DNA amplicons that can be conveniently detected. This approach was demonstrated for biosensing of a microRNA sequence and a bacterium.
(FR)
L'invention concerne une approche qui tire profit de l'amplification par cercle roulant (RCA) et d'une ADNzyme de clivage d'ARN (RCD) pour obtenir une amplification de signal massive pour une biodétection par l'intermédiaire d'un mécanisme de rétroaction croisée. Une réaction RCA génère des copies d'une RCD qui déclenche une cascade de réactions conçue pour générer des ensembles d'ADN supplémentaires pour la RCA. Lesdites actions de rétroaction croisée fonctionnent de manière autonome pour transformer de manière autonome des événements de reconnaissance moléculaire limités en quantités massives d'amplicons d'ADN qui peuvent être aisément détectés. Cette approche a été démontrée pour la biodétection d'une séquence de microARN et d'une bactérie.
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