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1. (WO2018161177) NUCLEIC ACID ENZYME-MEDIATED SIGNAL AMPLIFICATION FOR BIOSENSING
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/161177 International Application No.: PCT/CA2018/050294
Publication Date: 13.09.2018 International Filing Date: 12.03.2018
IPC:
C12Q 1/6844 (2018.01) ,C12Q 1/68 (2018.01) ,C12Q 1/682 (2018.01)
[IPC code unknown for C12Q 1/6844]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
[IPC code unknown for C12Q 1/682]
Applicants: MCMASTER UNIVERSITY[CA/CA]; 1280 Main Street West Hamilton, Ontario L8S 4L8, CA
Inventors: LI, Yingfu; CA
BRENNAN, John; CA
LIU, Meng; CA
Agent: BERESKIN & PARR LLP/S.E.N.C.R.L., S.R.L.; Scotia Plaza, 40 King Street West, 40th Floor Toronto, Ontario M5H 3Y2, CA
Priority Data:
62/469,77210.03.2017US
Title (EN) NUCLEIC ACID ENZYME-MEDIATED SIGNAL AMPLIFICATION FOR BIOSENSING
(FR) AMPLIFICATION DE SIGNAL, MÉDIÉE PAR UNE ENZYME, D'ACIDE NUCLÉIQUE POUR BIODÉTECTION
Abstract:
(EN) Described is an approach that takes advantage of rolling circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD) to achieve massive signal amplification for biosensing via a cross-feedback mechanism. An RCA reaction generates copies of an RCD that triggers a reaction cascade designed to generate additional DNA assemblies for RCA. These cross- feedback actions work autonomously to turn limited molecular recognition events into massive amounts of DNA amplicons that can be conveniently detected. This approach was demonstrated for biosensing of a microRNA sequence and a bacterium.
(FR) L'invention concerne une approche qui tire profit de l'amplification par cercle roulant (RCA) et d'une ADNzyme de clivage d'ARN (RCD) pour obtenir une amplification de signal massive pour une biodétection par l'intermédiaire d'un mécanisme de rétroaction croisée. Une réaction RCA génère des copies d'une RCD qui déclenche une cascade de réactions conçue pour générer des ensembles d'ADN supplémentaires pour la RCA. Lesdites actions de rétroaction croisée fonctionnent de manière autonome pour transformer de manière autonome des événements de reconnaissance moléculaire limités en quantités massives d'amplicons d'ADN qui peuvent être aisément détectés. Cette approche a été démontrée pour la biodétection d'une séquence de microARN et d'une bactérie.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)