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1. (WO2018161139) DYNAMIC CELLULAR CULTURE SYSTEM

Pub. No.:    WO/2018/161139    International Application No.:    PCT/BR2018/050061
Publication Date: Fri Sep 14 01:59:59 CEST 2018 International Filing Date: Thu Mar 08 00:59:59 CET 2018
IPC: C12M 3/04
B01L 3/00
C12N 5/071
Applicants: SOCIEDADE BENEFICENTE ISRAELITA BRASILEIRA HOSPITAL ALBERT EINSTEIN
Inventors: BEVILAQUA RANGEL, Érika
Title: DYNAMIC CELLULAR CULTURE SYSTEM
Abstract:
The present invention relates to a dynamic cellular culture system (1) comprising a culture plate (100) provided with at least one upper well (30) and at least one lower well (40) that are separated from one another by a semi-permeable membrane (50), the at least one upper well (30) receiving at least one upper cell culture medium (60) and cultivating therein a first cell type (80), at least one lower well (40) receiving at least one lower cell culture medium (70) and cultivating therein a second cell type (90), the first and second cell types (80, 90) being perfused, respectively, by at least one upper and lower cell culture medium (60, 70) simultaneously and independently of each other. The present invention also relates to a culture system comprising a PDMS chip (1000) provided with at least one upper micro-channel (301) and at least one lower micro-channel (401), the at least one upper and lower micro-channel (301, 401) being separated from one another by a 0 semi-permeable membrane (500), the at least one upper and lower micro-channel (301, 40) receiving at least one upper and lower cell culture medium (600, 700) and cultivating therein a first and a second cell type (800, 900), the first and second cell types (800, 900) being perfused, respectively, by at least one upper and lower cell culture medium (600, 700) simultaneously and independently of each other.