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1. (WO2018161046) MAGNETIC RESONANCE METHOD FOR DETECTING MOLECULAR BINDING WITH ENHANCED SENSITIVITY

Pub. No.:    WO/2018/161046    International Application No.:    PCT/US2018/020779
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Sat Mar 03 00:59:59 CET 2018
IPC: A61B 5/055
Applicants: THE JOHNS HOPKINS UNIVERSITY
KENNEDY KRIEGER INSTITUTE
Inventors: VAN ZIJL, Peter
YADAV, Nirbhay
Title: MAGNETIC RESONANCE METHOD FOR DETECTING MOLECULAR BINDING WITH ENHANCED SENSITIVITY
Abstract:
A method to detect transient binding of a substrate molecule of interest in solution to a molecular target includes selecting the substrate molecule of interest and the molecular target such that the substrate molecule of interest can transiently bind to the molecular target; placing one of a sample or a subject of interest in a magnetic resonance (MR) apparatus, the sample or the subject of interest containing the substrate molecule of interest so as to be in contact with the molecular target; providing magnetic labelling of non-exchangeable or slowly exchangeable MR sensitive nuclei of the substrate molecule of interest; receiving an MR signal from the MR sensitive nuclei of the solvent molecules using the MR apparatus; and analyzing the MR signal to obtain a quantity associated with the transient binding of the substrate molecule of interest to the molecular target.