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1. (WO2018160397) HIGHLY SPECIFIC CIRCULAR PROXIMITY LIGATION ASSAY

Pub. No.:    WO/2018/160397    International Application No.:    PCT/US2018/018859
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Wed Feb 21 00:59:59 CET 2018
IPC: C12Q 1/68
G01N 33/542
Applicants: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Inventors: PERSSON, Henrik H.J.
JALILI, Roxana
HORECKA, Joseph L.
DAVIS, Ronald W.
Title: HIGHLY SPECIFIC CIRCULAR PROXIMITY LIGATION ASSAY
Abstract:
Provided herein is a circular proximity ligation assay in which proximity-probes are employed as bridges to connect two free oligonucleotides via a dual ligation event, resulting in the formation of a circle. The circles are then quantified by, e.g., qPCR. The addition of an extra oligonucleotide is believed to enhance specificity by decreasing the probability of random background ligation events. In addition, circle formation may have selective advantages, as uncircularized DNA can be removed by a simple exonuclease treatment and it has streamlined the workflow by eliminating preamplification prior to qPCR.