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1. (WO2018160298) METHOD OF QUANTITATIVE MEASUREMENT OF PARTICLE CONTENT USING HYDRATED STATE IMAGING

Pub. No.:    WO/2018/160298    International Application No.:    PCT/US2018/015263
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Fri Jan 26 00:59:59 CET 2018
IPC: C07K 14/005
C07K 14/01
C07K 14/015
G01N 15/02
Applicants: INTELLIGENT VIRUS IMAGING INC.
Inventors: COLOMB-DELSUC, Mathieu
HAAG, Lars
NORDSTRÖM, Rickard
RYNER, Martin
Title: METHOD OF QUANTITATIVE MEASUREMENT OF PARTICLE CONTENT USING HYDRATED STATE IMAGING
Abstract:
The method is for quantitative measurement of particle content using hydrated state imaging such as CryoTEM. A sample (100) of virus-like particles (VLPs) or virus particles is provided. Preferably, the sample (100) is rapidly frozen into a cryogenic liquid at a cryogenic temperature. While at the cryogenic temperature, the particle content of each VLP in the frozen sample is observed in the CryoTEM. An amount of the particle content of the VLPs is determined to assess whether the VLPs are empty or not.