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1. (WO2018159842) PRODUCTION METHOD FOR DARBEPOETIN COMPOSITION, AND CULTURE METHOD FOR DARBEPOETIN-PRODUCING CELLS

Pub. No.:    WO/2018/159842    International Application No.:    PCT/JP2018/008156
Publication Date: Sat Sep 08 01:59:59 CEST 2018 International Filing Date: Sat Mar 03 00:59:59 CET 2018
IPC: C12P 21/02
C07K 1/18
C07K 1/20
C07K 1/34
C07K 14/505
C12N 5/10
Applicants: KYOWA HAKKO KIRIN CO., LTD.
協和発酵キリン株式会社
Inventors: TSUKAHARA Masayoshi
塚原 正義
YAMAMOTO Koichi
山本 耕一
ISHIHARA Takashi
石原 尚
HOSONO Mareto
細野 眞礼登
NAKAGAWA Taishiro
中川 泰志郎
OKUMURA Naofumi
奥村 直史
ISODA Tomoko
磯田 智子
Title: PRODUCTION METHOD FOR DARBEPOETIN COMPOSITION, AND CULTURE METHOD FOR DARBEPOETIN-PRODUCING CELLS
Abstract:
The purpose of the present invention is to provide a method for culturing darbepoetin-producing cells and thereby producing a darbepoetin composition, wherein the method increases the total amount of the darbepoetin composition produced and increases the sialic acid content of each darbepoetin molecule excreted into a culture solution by the cells. The present invention relates to a production method for a darbepoetin composition. The production method includes: a first step for culturing darbepoetin-producing cells in a culture medium to which a plant-derived protein hydrolysate has been added and obtaining a culture solution; and a second step for collecting the darbepoetin composition from the culture solution obtained in step 1.